195. Bo Zhu*$, Zhe Du$, Yancen Dai, Tetsuya Kitguchi, Sebastian Behrens, Burckhard Seelig* "Nanodroplet-based reagent delivery into water-in-fluorinated-oil droplets" Biosensors 13, 768 (2023). [DOI] $Equal contributions. Open Access
194. Sohei Hiasa, Takeru Fujimori, Saki Aiki, Hiroshi Ueda, Takashi Tsuboi and Tetsuya Kitaguchi "Development of green fluorescent protein-based cAMP indicators for covering a wide range of cAMP concentrations" RSC Adv 13, 15514–15520 (2023). [DOI] Open Access
193. Yoshihiro Ito, Ryuichi Sasaki, Sayaka Asari, Takanobu Yasuda, Hiroshi Ueda, Tetsuya Kitaguchi "Efficient microfluidic screening method using a fluorescent immunosensor for recombinant protein secretions" Small 19, 2207943 (2023). [DOI]
192. Satoshi Okada, Yuka Muto, Bo Zhu, Hiroshi Ueda, Hiroyuki Nakamura "Development of a peptide sensor derived from human ACE2 for fluorescence polarization assays of the SARS-CoV-2 receptor binding domain" Anal. Chem. 95, 6198–6202 (2023). [DOI]
191. Abdul Q. Rani, Bo Zhu, Hiroshi Ueda and Tetsuya Kitaguchi "Recent progress in homogeneous immunosensors based on fluorescence or bioluminescence using antibody engineering" Analyst 148, 1422-1429 (2023). (Review) [DOI]
190. Donnell White III, Lothar Lauterboeck, Parnia Mobasheran, Tetsuya Kitaguchi, Antoine H. Chaanine and Qinglin Yang "Real-time visualization of cytosolic and mitochondrial ATP dynamics in response to metabolic stress in cultured cells" Cells 12, 695 (2023). [DOI] Open Access
189. Kazuki Harada, Maoko Takashima, Tetsuya Kitaguchi and Takashi Tsuboi "Time-dependent shift in docking dynamics of glucagon-like peptide-granules from enteroendocrine L cell line GLUTag cells" FEBS Lett. 597, 657-671 (2023). [DOI] Open Access
188. J. Su$, B. Zhu$, A. Inoue, H. Oyama, I. Morita, J. Dong, T. Yasuda, Y. Sugita-Konishi, T. Kitaguchi, N. Kobayashi, S. Miyake and H. Ueda "The Patrol Yeast: a new biosensor armed with antibody-receptor chimera detecting a range of toxic substances associated with food poisoning" Biosens. Bioelectron. 219, 114793 (2023). [DOI] $Equal contributions. Open Access
187. B. Zhu$, N. Nosaka$, S. Kanamaru, J. Dong, Y. Dai, A. Inoue, Y. Yang, K. Kobayashi, T. Kitaguchi, H. Iwasaki, R. Koike, K. Wakabayashi and H. Ueda "Rapid and sensitive SARS-CoV-2 detection using a homogeneous fluorescent immunosensor Quenchbody with crowding agents" Analyst 147, 4971-4979 (2022). [DOI] $Equal contributions. Selected as an Analyst HOT Article 2022 and a Back Cover!
<- Altimetric score 186. H. Li$, B. Zhu$, B. Li, L. Chen, X. Ning, H. Dong, J. Liang, X. Yang, J. Dong and H. Ueda "Isolation of a Human SARS-CoV-2 Neutralizing Antibody from a Synthetic Phage Library and its Conversion to Fluorescent Biosensors" Sci. Rep. 12, 15496 (2022). [DOI] Open Access $Equal contributions.
185. M. Takizawa, Y. Osuga, R. Ishida, M. Mita, K. Harada, H. Ueda, T. Kitaguchi and T. Tsuboi "Development of a red fluorescent protein-based cGMP indicator applicable for live-cell imaging" Communications Biology 5, 833 (2022). [DOI] Open Access
184. Y. Dai, Y. Sato, B. Zhu, T. Kitaguchi, H. Kimura, F.J. Ghadessy and H. Ueda "Intra Q-body: an antibody-based fluorogenic probe for intracellular proteins that allows live cell imaging and sorting" Chemical Science 13, 9739-9748 (2022). [DOI][EurekAlert!] Open Access
<- Altimetric score 183. B. Zhu$, C. Qian$, H. Tang, T. Kitaguchi and H. Ueda "Creating a thermostable beta-glucuronidase switch for homogeneous immunoassay by disruption of conserved salt bridges at diagonal interfaces" Biochemistry 62, 309–317 (2023). [DOI] [PDF–ACS Articles on Request] $Equal contributions
182. H.-O. Yun, H. Ueda and H.-J. Jeong "Development of a spacer-optimized Quenchbody against tumor necrosis factor alpha" Biotechnol. Bioproc. Eng. 27, 820-830 (2022). [DOI]
181. I. Morita, Y. Kiguchi, H. Oyama, K. Yamaki, N. Sakio, K. Kashiwabara, Y. Kuroda, A. Ito, A. Yokota, N. Ikeda, A. Takeuchi, C. Tode, R. Kikura-Hanajiri, H. Ueda, S. Numazawa, T. Yoshida and N. Kobayashi "Derivatization-assisted immunoassays: application for group-specific detection of potent methamphetamine and amphetamine enantiomers" Anal. Methods 14, 2745-2753 (2022). [DOI] Selected as a Hot Article!
180. J. Liang, H. Dong, H. Wang, Z. Yi, G. Jiang, T. Inagaki, C.E. Gomez-Sanchez, J. Dong* and H. Ueda* "Creation of a Quick and Sensitive Fluorescent Immunosensor for Detecting the Mineralocorticoid Steroid Hormone Aldosterone" J. Steroid Biochem. Mol. Biol. 221, 106118 (2022). [DOI] [Share Link]
179. Y. Ohmuro-Matsuyama, T. Furuta, H. Matsui, M. Kanai and H. Ueda "Miniaturization of the bright light-emitting luciferase ALuc: picALuc" ACS Chem. Biol. 17, 864-872 (2022). [DOI]
<- Altimetric score 178. A. Inoue, T. Yasuda, B. Zhu, T. Kitaguchi, A. Murakami and H. Ueda "Evaluation and Selection of Potent Fluorescent Immunosensors by Combining Fluorescent Peptide and Nanobodies Displayed on Yeast Surface" Sci. Rep. 11, 22590 (2021). [DOI] Open Access
177. H. Li, X. Li, L. Chen, B. Li, H. Dong, H. Liu, X. Yang, H. Ueda* and J. Dong* "Quench-release-based Fluorescent Immunosensor for the Rapid Detection of Tumor Necrosis Factor-alpha" ACS Omega 6, 46, 31009-31016 (2021). [DOI] Open Access
176. Y. Ohmuro-Matsuyama, K. Gomi, T. Shimoda, H. Yamaji and H. Ueda "Improving the stability of protein–protein interaction assay FlimPIA using a thermostabilized firefly luciferase" Front. Bioeng. Biotechnol. 9, 778120 (2021). [DOI] Open Access
175. S. Sato, M. Matsumura, H. Ueda and H. Nakamura "Preparation of an antigen-responsive fluorogenic immunosensor by tyrosine chemical modification of the antibody complementarity determining region" Chem. Commun. 57, 9760-9763 (2021). [DOI]
174. X. Ning, T. Yasuda, T. Kitaguchi and H. Ueda "Construction of fluorescent immunosensor Quenchbody for detecting His-tagged recombinant proteins produced in bioprocess" Sensors 21, 4993 (2021). [DOI] Open Access
173. T. Yasuda, A. Inoue, T. Kitaguchi and H. Ueda "Rapid construction of fluorescence quenching-based immunosensor Q-bodies using α-helical coiled-coil peptides" Chem. Commun. 57, 8206-8209 (2021). [DOI]
172. M. Mita, I. Sugawara, K. Harada, M. Ito, M. Takizawa, K. Ishida, H. Ueda, T. Kitaguchi and T. Tsuboi "Single fluorescent protein-based red glucose indicators visualize intracellular glucose dynamics" Cell Chem. Biol. 28, 1-11 (2021). [DOI]
171. Y. Liang, Y. Wang, F. Wang, J. Li, C. Wang, J. Dong, H. Ueda, Z. Xiao, Y. Shen, Z. Xu and H. Wang "An Enhanced Open Sandwich Immunoassay by Molecular Evolution for Noncompetitive Detection of Alternaria Mycotoxin Tenuazonic Acid" Food Chem. 361, 130103 (2021).[DOI]
170. R. Takahashi$, T. Yasuda$, Y. Ohmuro-Matsuyama and H. Ueda "BRET Q-Body : A Ratiometric Quench-based Bioluminescent Immunosensor Made of Luciferase–Dye–Antibody Fusion with Enhanced Response" Anal. Chem. 93, 21, 7571-7578 (2021). [DOI] [PDF–ACS Articles on Request] [EurekAlert!] $Equal contributions Adopted as Supplemenatry Journal Cover
<- Altimetric score 169. Y. Ohmuro-Matsuyama, T. Kitaguchi, H. Kimura and H. Ueda "A simple fluorogenic cellular assay for histone deacetylase inhibitors based on split-YFP and intrabodies" ACS Omega 6, 10039-10046 (2021). [DOI]
<- Altimetric score168. Okuyama, Hiroto; Tamaki, Takanori; Oshiba, Yuhei; Ueda, Hiroshi; Yamaguchi, Takeo "Numerical Modeling for Sensitive and Rapid Molecular Detection by Membrane-Based Immunosensors" Anal. Chem. 93 , 7210-7219 (2021).[DOI]
167. Yoshikata Harada, Yuki Ohmuro-Matsuyama, Mika Tsuna and Hiroshi Ueda "An Open Sandwich Immunochromatography for Non-competitive Detection of Small Antigens" Anal. Sci. 37, 455-459 (2021).[DOI] Selected as a Hot Paper!
166. K. Harada, T. Chihara, Y. Hayasaka, M. Mita, M. Takizawa, K. Ishida, M. Arai, S. Tsuno, M. Matsumoto, T. Ishihara, H. Ueda, T. Kitaguchi and T. Tsuboi "Green Fluorescent Protein-Based Lactate and Pyruvate Indicators Suitable for Biochemical Assays and Live Cell Imaging" Sci. Rep. 10, 19562 (2020). [DOI] Open Access
165. A. Inoue, Y. Ohmuro-Matsuyama, T. Kitaguchi and H. Ueda "Creation of a nanobody-based fluorescent immunosensor mini Q-body for rapid signal-on detection of small hapten methotrexate" ACS Sens. 5(11), 3457–3464 (2020). [DOI] [PDF–ACS Articles on Request]
164. L. Chen, R. Tan, Y. Zhou, L. Zhang, S. Zhanga, X. Li, Y. Cong, H. Li, P. Sun, H. Ueda and J. Dong "Development of an Open Sandwich ELISA for the Detection of Microcystin-LR" Microchem. J. 158, 105325 (2020).[DOI]
163. J. Dong, C. Miyake, T. Yasuda, H. Oyama, I. Morita, T. Tsukahara, M. Takahashi, H.-J. Jeong, T. Kitaguchi, N. Kobayashi and H. Ueda "PM Q-probe: a fluorescent binding protein that converts many antibodies to a fluorescent biosensor" Biosens. Bioelectron. 165, 112425 (2020). [DOI]
.jpg)
162. H.-J. Jeong, J. Dong, C.-H. Yeom and H. Ueda "Synthesis of Quenchbodies for One-Pot Detection of Stimulant Drug Methamphetamine" Methods Protoc. 3(2), 43 (2020). [DOI] Open Access
161. Han C, Choi BK, Kim S-H, Sim S-J, Han S, Park B, Tsuchiya Y, Takahashi M, Kim YH, Eom H-S, Kitaguchi T, Ueda H and Kwon BS, "Polymorphic region-specific antibody for evaluation of affinity-associated profile of chimeric antigen receptor" Mol. Ther.: Oncolytics 17, 293-305 (2020). [DOI] Open Access
160. S. Sato, M. Matsumura, T. Kadonosono, S. Abe, T. Ueno, H. Ueda and H. Nakamura "Site-Selective Protein Chemical Modification of Exposed Tyrosine Residues Using Tyrosine Click Reaction" Bioconj. Chem. 31, 1417-1424 (2020). [DOI] [PDF–ACS Articles on Request]
159. J. Dong$, Y. Oka$, H.-J. Jeong, Y. Ohmuro-Matsuyama and H. Ueda "Detection and destruction of HER2-positive cancer cells by Ultra Quenchbody-siRNA complex" Biotechnol. Bioeng. 117, 1259-1269 (2020). [DOI] $Equal contributions
158. Y. Kanai$, Y. Ohmuro-Matsuyama$, M. Tanioku, S. Ushiba, T. Ono, K. Inoue, T. Kitaguchi, M. Kimura, H. Ueda* and K. Matsumoto "Graphene Field Effect Transistor-Based Immunosensor for Ultrasensitive Noncompetitive Detection of Small Antigens" ACS Sens. 5, 24-28 (2020).[DOI][PDF–ACS Articles on Request] $Equal contributions
B. Zhu$, R. Gan$, M. D. Cabezas, T. Kojima, R. Nicol, M. C. Jewett and H. Nakano "Increasing cell-free gene expression yields from linear templates in Escherichia coli and Vibrio natriegens extracts by using DNA-binding proteins" Biotechnol. Bioeng. 117, 3849-3857 (2020). [DOI] $Equal contributions
総説・著書
中村昌文，上田 宏 "研究会へようこそ！生物化学的測定研究会ー環境測定に生体反応を生かす" 化学 77 (6), 17-19, 2022 (化学同人, 京都). [Link]
上田 宏 "抗体の遺伝子工学と新規免疫測定法への応用" 先端の分析法, 2nd Ed. (eds. 小澤, 北森, 中村, 藤浪, 宮村) 814-819 (NTS, 東京, 2022). [Link]
上田 宏 "免疫測定法の高性能化" 先端の分析法, 2nd Ed. (eds. 小澤, 北森, 中村, 藤浪, 宮村) 820-822 (NTS, 東京, 2022). [Link]
上田 宏 "東日本支部・温故知新（18） 東京工業大学 生命理工学院・同 科学技術創成研究院化学生命科学研究所における生物工学研究" 生物工学会誌 99 (7), 378-81 (2021).[DOI]
Hiroshi Ueda, Yancen Dai, Farid Ghadessy "Visualizing intracellular target antigens in live cells" Trends in Cell Biology 33, 277-279 (2023).[DOI]
Mita Marie, Wongso Devina, Ueda Hiroshi, Tsuboi Takashi, Kitaguchi Tetsuya "Development of a Single Fluorescent Protein-Based Green Glucose Indicator by Semirational Molecular Design and Molecular Evolution" Live Cell Imaging, Methods Mol. Biol. 2274, 89-100 (2021).[DOI]
Hiroshi Ueda and Jinhua Dong "Biosensors: Homogeneous Detection" Reference Module in Biomedical Sciences (2021).[DOI]
上田 宏 "抗体関連タンパク質の蛍光修飾によるバイオセンサー化" 生物工学会誌 99, 168-171 (2021).[DOI] Open Access
Jinhua Dong and Hiroshi Ueda "Quenchbody" Encyclopedia 8932 (2021).[Link] Open Access
Jinhua Dong and Hiroshi Ueda"Recent Advances in Quenchbody, a Fluorescent Immunosensor" Sensors 21, 1223 (2021).[DOI] Open Access
上田 宏，"新型コロナと診断と生物化学工学" 化学工学会バイオ部会ニュースレター 51, 1-2 (2020).[Link]
董 金華，上田 宏， "ヒト由来新型コロナウイルス中和抗体に共通する特徴" 実験医学 38(18) 11月号, 3092-3 (2020).[Link]
招待講演
°Hiroshi Ueda, Bo Zhu, Jinhua Dong “Isolation of recombinant human antibody for the prevention and rapid diagnosis of COVID-20” Joint SBJ Meeting with Indonesia, Philippines, and Thailand, Online (10/21, 2022) [Link]
○上田 宏, 蘇 九龍, 朱 博, 井上 暁人, 大山 浩之, 森田 いずみ, 董 金華, 小西 良子, 北口 哲也, 小林 典裕, 三宅 司郎, “各種食中毒から生活者を守る「パトロール酵母」の開発” 第74回日本生物工学会大会, オンライン開催(10/18, 2022) [Link]
上田 宏 "タンパク質を使ってものを測る", 生物工学会セミナー, 都立戸山高等学校 (12/18, 2021)
Hiroshi Ueda, Akihito Inoue, Takanobu Yasuda, and Akikazu Murakami "Evaluation and Selection of Potent Fluorescent Immunosensors by Combining Fluorescent Peptide and Nanobodies Displayed on Yeast Surface", Virtual International Symposium on Immunoanalytical Technologies for Environmental Monitoring and Human Health, オンライン開催 (12/10, 2021) [Link]
上田 宏 "発光酵素の蛋白質工学とそのセンサー化", 生物発光化学発光研究会第36回学術講演会, オンライン開催 (11/27, 2021) [Link]
上田 宏 "食中毒から生活者を解放する人工抗体提示パトロール酵母", JST 未来社会創造事業 新技術説明会，オンライン開催 (5/28, 2021) [Link]
○上田 宏、有坂 亮汰、朴 俊泰、董 金華、 柴田 貴広、北口 哲也、内田 浩二 "ゲノム編集法による酸化脂質類の非競合的免疫測定系の構築", 第74回 日本酸化ストレス学会シンポジウム Ss2-1 "酸化ストレス疾患病態解析における分析技術の新展開"，オンライン開催 (5/20, 2021) [Link]
Hiroshi Ueda "A fluorescent immunosensor for diagnosing the corona for one individual, one minute and one dollar", 2nd Tokyo Tech International Open Innovation Symposium 2021, online (2/8, 2021) [Link]
Hiroshi Ueda "Creation of a Nanobody-Based Fluorescent Immunosensor Mini Q-body for Rapid Signal-On Detection of Small Hapten Methotrexate", The 3rd International Conference on Nanobody for Immunological Analysis and Application, online (12/5, 2020)
上田 宏 "１人，１分，１ドルで判定可能なSARS-CoV-2診断のための取り組み",
東京工業大学科学技術創成研究院 研究院公開，オンライン開催 (12/3, 2020) [Link]
上田 宏，蘇 九龍 "人工細胞系を用いたデジタル免疫測定への挑戦",
日本生物工学会生物工学Webシンポジウム2020，オンライン開催 (9/2, 2020) [Link]
上田 宏 "タンパク質工学的手法による各種蛍光・発光免疫センサーの開発",
電気学会 光・量子デバイス研究会「医療・バイオ応用を目指したナノ構造・ナノ界面」, 西播磨地場産業センター，姫路 (1/8, 2020)
