Y. Cong, H. Dong, X. Wei, L. Zhang, J. Bai, J. Wu, J.X. Huang, Z. Gao, H. Ueda and J. Dong "A novel murine antibody and an open sandwich immunoassay for the detection of clenbuterol"
Ecotoxicol. Environ. Saf.
182, 109473 (2019).[DOI]
C.-I. Chung, Y. Sato, Y. Ohmuro-Matsuyama, S. Machida, H. Kurumizaka, H. Kimura and H. Ueda "Intrabody-based FRET probe to visualize endogenous histone acetylation" Sci. Rep.
9, 10188 (2019). [DOI]
J. Su, C. Beh, Y. Ohmuro-Matsuyama, T. Kitaguchi, S. Hoon and H. Ueda "Creation of stable and strictly regulated enzyme switch for signal-on immunodetection of various small antigens"
J. Biosci. Bioeng.
in press, (2019).[DOI]
R. Dale, Y. Ohmuro-Matsuyama, H. Ueda and N. Kato "Non-steady state analysis of enzyme kinetics in real time elucidates substrate association and dissociation rates: demonstration with analysis of firefly luciferase mutants"
2695-2702 (2019). [DOI]
M. Mita, M. Ito, K. Harada, I. Sugawara, H. Ueda, T. Tsuboi and T. Kitaguchi "Green fluorescent protein-based glucose indicators report glucose dynamics in living cells"
4821-4830 (2019). [DOI]
H.J. Jeong, J. Dong and H. Ueda "Single-step detection of influenza virus hemagglutinin using bacterially produced Quenchbodies"
52 (2019). [DOI]
A. Sasao, M. Takaki, H.-J. Jeong, K. Yonemitsu, Y. Ohtsu, H. Tsutsumi, S. Furukawa, H. Morioka, H. Ueda and Y. Nishitani "Development of a fluvoxamine detection system using a Quenchbody, a novel fluorescent biosensor"
Drug Test. Anal.
11, 601-609 (2019). [DOI]
Y. Ohmuro-Matsuyama, T. Yamashita, K. Gomi, H. Yamaji and H. Ueda "Evaluation of protein-ligand interactions using the luminescent interaction assay FlimPIA with streptavidin-biotin linkage" Anal. Biochem.
, 61-66 (2018). [DOI]
J. Dong, R. Fujita, T. Zako and H. Ueda "Construction of Quenchbodies to detect and image amyloid β oligomers"
S. Zhao, J. Dong, H.-J. Jeong, K. Okumura and H. Ueda "Rapid detection of the neonicotinoid insecticide imidacloprid using a quenchbody assay"
Anal. Bioanal. Chem.
J. Su, D. Jinhua, T. Kitaguchi, Y. Ohmuro-Matsuyama and H. Ueda "Noncompetitive homogeneous immunodetection of small molecules based on beta-glucuronidase complementation" Analyst
143, 2096-2101 (2018).[DOI]
Y. Ohmuro-Matsuyama and H. Ueda "Homogeneous noncompetitive luminescent immunodetection of small molecules by ternary protein fragment complementation" Anal. Chem.
(2018).[DOI] [PDF–ACS Articles on Request]
H.-J. Jeong, T. Kawamura, M. Iida, Y. Kawahigashi, M. Takigawa, Y. Ohmuro-Matsuyama, C.-I. Chung, J. Dong, M. Kondoh and H. Ueda "Development of a Quenchbody for the detection and imaging of the cancer-related tight-junction-associated membrane protein claudin" Anal. Chem.
(2017). [DOI] [PDF–ACS Articles on Request]
M. Okochi, M. Muto, K. Yanai, M. Tanaka, T. Onodera, J. Wang, H. Ueda and K. Toko "Array-based rational design of short peptide probe derived from anti-TNT monoclonal antibody" ACS Comb. Sci.
H.-J. Jeong, K. Matsumoto, S. Itayama, K. Kodama, R. Abe, J. Dong, M. Shindo and H. Ueda
Construction of dye-stapled Quenchbody by photochemical crosslinking to antibody nucleotide-binding site" Chem. Comm.
10200-10203 (2017). [DOI]
Y. Ohmuro-Matsuyama, T. Yamashita, H. Lin, H. Yamaji and H. Ueda "Improved sensitivity of firefly luminescent intermediate-based protein interaction assay using Ser 440 mutant with lower adenylation activity" Luminescence
D. Wongso, J. Dong, H. Ueda and T. Kitaguchi "Flashbody: a next generation Fluobody with fluorescence intensity enhanced by antigen binding" Anal. Chem.
J. Dong, M.
Shichiri, C.-I. Chung, T. Shibata, K. Uchida, Y. Hagihara,
Y. Yoshida and H. Ueda "An Open Sandwich immunoassay for
detection of 13(R,S)-Hydroxy-9(E),11(E)-octadecadienoic
142, 787-793 (2017).[DOI]
T. Kojima, J. Dong, H. Ohashi and H. Ueda "One-pot
construction of Quenchbodies using antibody-binding
proteins" Anal. Methods
137. C.-I. Chung, R. Makino, Y.
Ohmuro-Matsuyama and H. Ueda "Development of a fluorescent
protein-antibody FRET probe for the detection and imaging
of osteocalcin" J. Biosci.
123, 272-276 (2017).
H. Ohashi, T.
Matsumoto, H.-J. Jeong, J. Dong, R. Abe and H. Ueda
"Insight into the working mechanism of Quenchbody:
Transition of the dye around antibody variable region that
fluoresces upon antigen binding" Bioconj. Chem.
2248-2253 (2016). [DOI] [PDF–ACS Articles on Request]
135. H. Iwai, M. Kojima-Misaizu, J. Dong, and H. Ueda "Creation of a ligand-dependent enzyme by fusing circularly permuted antibody variable region domains" Bioconj.
27, 868-873 (2016). [ DOI] [Abstract] [PDF–ACS Articles on Request]
Y. Ohmuro-Matsuyama, K. Mori, H. Hamada, H. Ueda and H. Yamaji "Electrostatic engineering of the interface between heavy and light chains promotes antibody Fab fragment production"
(3), 469-475 (2017).[DOI]
Dale, Y. Ohmuro-Matsuyama, H. Ueda and N. Kato
"Mathematical model of the firefly luciferase
complementation assay reveals a non-linear relationship
between the detected luminescence and the affinity of the
protein pair being analyzed" PLoS
J. Dong, H.J. Jeong and H. Ueda "Preparation of Quenchbodies by protein transamination reaction" J. Biosci.
122, 125-130 (2016). [DOI][Abstract]
Jeong, T. Kawamura, J. Dong and H. Ueda "Q-bodies from
recombinant single-chain Fv fragment with better yield and
expanded palette of fluorophores" ACS
1, 91-99 (2016).[DOI]
Kurihara*, Y. Ohmuro-Matsuyama*, K. Ayabe, T. Yamashita, H.
Yamaji and H. Ueda "Ultra sensitive firefly
luciferase-based protein-protein interaction assay
(FlimPIA) attained by hinge region engineering and
optimized reaction conditions" Biotechnol.
11, 91-99 (2016). [Abstract][DOI] *equal
Komatsu, J. Dong, H. Ueda and R. Arai "Crystal structure of
Fab fragment of an anti-osteocalcin C-terminal peptide
antibody KTM219" Photon
Factory Act. Rep. 2014 Part B, 205 (2015).
128. J. Gholami, M. Manteghian, A. Badiei, M. Javanbakht
and H. Ueda "Label free detection of vitamin B12 based on
fluorescence quenching of graphene oxide nanolayer"
Nanotubes and Carbon Nanostructures
(2015).[DOI][Free Full Text]
127. J. Gholami, M. Manteghian, A. Badiei, H. Ueda and M.
Javanbakht "N-butylamine functionalized graphene oxide for
detection of Iron(III) by photoluminescence
31 , 229-235 (2016).
J. Dong*, T. Kojima*, H. Ohashi and H. Ueda "Optimal fusion
of antibody binding domains resulted in higher affinity and
wider specificity" J. Biosci.
(2015). *equal contributions [DOI][Abstract][Free download~11/27]
Jeong, S. Itayama and H. Ueda "A signal-on fluorosensor
based on quench-release principle for sensitive detection
of antibiotic rapamycin" Biosensors
C.I. Chung, R. Makino, J. Dong and H. Ueda "Open flower
fluoroimmunoassay: a general method to make fluorescent
protein-based immunosensor probes" Anal.
(2015).[Abstract][DOI][ACS Articles on Request]
123. H. Veisi,
J. Gholami, H. Ueda, P. Mohammadi and M. Noroozi
"Magnetically palladium catalyst stabilized by
active and reusable catalyst for Suzuki coupling
reactions" J. Mol.
Catal. A: Chemical
Hayashi, Y. Tomozoe, K. Nagai, K. Matsuba, M. Mitsumori, Y.
Hiraishi, T. Matsumura, H. Ueda and N. Kamiya "Optimization
of a fusion protein expression system using human cell
lines for creating a practical immunoassay reagent"
121. H.-J. Jeong and
H. Ueda "Strategy for making a superior Quenchbody to
proteins: effect of the fluorophore position"
Sasajima, Y. Kohama, M. Kojima-Misaizu, N. Kurokawa, Y.
Hara, J. Dong, M. Ihara and H. Ueda "Simultaneous retention
of thermostability and specific activity in chimeric human
alkaline phosphatases" Mol.
Abe, H.J. Jeong, D. Arakawa, J.H. Dong, H. Ohashi, R.
Kaigome, F. Saiki, K. Yamane, H. Takagi and H.Ueda "Ultra
Q-bodies : quench-based antibody probes that utilize
dye-dye interactions with enhanced antigen-dependent
Ohmuro-Matsuyama, Y. Hara and H. Ueda "Improved
protein-protein interaction assay FlimPIA by the entrapment
of luciferase conformation" Anal.
Jinhua Dong and Hiroshi Ueda "Open sandwich immunoassay and its applications" in Advances in Medicine and Biology, 125. (ed. L.V. Berhardt) pp. 123-159 (Nova Science Publishers Inc., New York; 2017). [PDF (PW:392062)]
H. Iwai, M.
Kojima-Misaizu, J. Dong and H. Ueda "Creation of
antigen-dependent β-lactamase fusion protein tethered by
circularly permuted antibody variable domains"
149-165 (2017). [DOI]
Ohmuro-Matsuyama and H. Ueda "Ultrasensitive Firefly
luminescent intermediate-based Protein-protein Interaction
Assay (FlimPIA) based on the functional complementation of
mutant firefly luciferases" Methods Mol.
119-130 (2017). [DOI]
Jinhua Dong and Hiroshi Ueda "ELISA-type assays of trace biomarkers using microfluidic methods" WIREs Nanomed. Nanobiotechnol. e1457 (2017). [Abstract]
Y. Ohmuro-Matsuyama and H. Ueda "A protein-protein interaction assay FlimPIA based on the functional complementation of mutant firefly luciferases"
" Methods Mol. Biol.
Hiroshi Ueda, "Improving Medical Care with Engineering" SCIENTIA October issue "Innovations in Healthcare", Knowledge Translation Media, Gloucester, UK (2015). [PDF]
H. Ueda and J.
Dong "From Fluorescence Polarization to Quenchbody : Recent
Progress in Fluorescent Reagentless Biosensors Based on
Antibody and Other Binding Proteins" Biophys.
Biochim. Acta - Proteins and Proteomics
Invited speaks (English only)
Ueda “How can we transform monofunctional proteins to
Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences,
Nanyang Technological University, Singapore
Hiroshi Ueda, Yuya Oka, Chan-I Chung, Hee-Jin Jeong, Jinhua Dong “Antibody-based Fluorescent Probes: How Can We Convert Antigen Binding Signal to Fluorescence Signal?”, Third FB3 (Fluorescent Biomolecules and their Building Blocks) Conference, Tianjin University, Tianjin, People's Republic of China (7/8, 2016)
Hiroshi Ueda “Development of biosensor proteins utilizing fluorescence quenching and bioluminescent reactions”, 6th International Conference on Bimolecular Engineering, Society for Biological Engineering, AIChE, Grand Hyatt Singapore, Singapore (1/5, 2016)
Hiroshi Ueda, Hideki Yamaji, Yuki Ohmuro-Matsuyama. "Hinge region engineering of firefly luciferases for robust protein-protein interaction assay FlimPIA", Pacifichem 2015 (Honolulu, HI) (12/16, 2015)
H. Ueda “Quenchbodies - Quench-based antibody probes that emit antigen-dependent fluorescence”, 1st International Conference and Exhibition on Antibodies, Hilton Birmingham Metropole, Birmingham, UK (8/10, 2015)
H. Ueda “Development of biosensor proteins utilizing fluorescence quenching and bioluminescent reactions”, 42nd Annual Meeting and International Symposium of the Korean Society for Microbiology and Biotechnology (KMB2015), Gyeongju Hwabaek International Convention Center (HICO), Gyeongju, Korea (6/24, 2015)
H. Ueda “How can
we transform monofunctional proteins to sensors?”,
A*Star-JST Joint Workshop “Development of Fundamental
Technology for Biodevices Enabling Dynamic Analysis and
Control of Cells” Exploration Theatrette, Matrix Level 4,
Biopolis, Singapore (1/12, 2015)
H. Ueda “Creation of biosensors based on the engineering of
antibody and bioluminescent enzyme”, 4th International
Symposium on Interdisciplinary Domain Research, Shizuoka
Shizuoka (12/1, 2014)
H. Ueda “How can
we transform uni-functional proteins to sensors?”,
YABEC, Chayi, Taiwan
(11/7, 2014), selected as a plenary speaker.
* The numbers
are colored according to the research projects as follow: