Publications:2001-03

Original papers

51. Aburatani, T., K. Sakamoto, K. Masuda, K. Nishi, H. Ohkawa, T. Nagamune and H. Ueda. "A general method to select antibody fragments suitable for noncompetitive detection of monovalent antigens." Anal. Chem. 75(16), 4057-4064 (2003) [Abstract][PDF]

50. Kamiya, N., T. Takazawa, T. Tanaka, H. Ueda and T. Nagamune. "Site-specific crosslinking of functional proteins by transglutamination."
Enzyme Microb. Tech. 33, 492-496 (2003)

49. Kawahara, M., H. Ueda, S. Morita, K. Tsumoto, I. Kumagai and T. Nagamune. "Bypassing antibiotic selection: positive screening of genetically modified cells with an antigen-dependent proliferation switch." Nucleic Acids Res. 31(7), e32 (2003) [Abstract][PDF]

48. Kitayama, A., H. Yoshizaki, Y. Ohmiya, H. Ueda* and T. Nagamune. "Creation of a thermostable firefly luciferase with pH-insensitive luminescent color." Photochem. Photobiol. 77(3), 333-338 (2003) [Abstract]

47. Ueda, H., T. Yokozeki, R. Arai, K. Tsumoto, I. Kumagai and T. Nagamune. "An optimized homogeneous noncompetitive immunoassay based on the antigen-driven enzymatic complementation." J. Immunol. Methods 279, 209-218 (2003) [Abstract]

46. Zako, T., K. Ayabe, T. Aburatani, N. Kamiya, A. Kitayama, H. Ueda* and T. Nagamune. "Luminescent and substrate binding activities of firefly luciferase N-terminal domain." Biochim. Biophys. Acta 1649, 183-189 (2003) [Abstract]

45.
Aburatani, T., H. Ueda* and T. Nagamune. "Importance of a CDR H3 basal residue in VH/VL interaction of human antibodies." J. Biochem. 132(5), 775-782 (2002) [Abstract]

44. Arai, R., H. Nakagawa, A. Kitayama, H. Ueda and T. Nagamune. "Detection of protein-protein interaction by bioluminescence resonance energy transfer from firefly luciferase to red fluorescent protein." J. Biosci. Bioeng. 94(4), 362-364 (2002) [Abstract] [PDF]

43. Kawahara, M., H. Ueda, K. Tsumoto, I. Kumagai, W. Mahoney and T. Nagamune. "Selection of highly productive mammalian cells based on an inducible growth advantage using an antibody/receptor chimera." J. Biosci. Bioeng. 93(4), 399-404 (2002) [Abstract] [PDF]

42. Ohiro, Y., R. Arai, H. Ueda and T. Nagamune. "A homogeneous and noncompetitive immunoassay based on the enhanced fluorescence resonance energy transfer by leucine zipper interaction." Anal. Chem. 74(22), 5786-5792 (2002) [Abstract]

41. Yamakawa, Y., H. Ueda*, A. Kitayama and T. Nagamune. "Rapid homogeneous immunoassay of peptides based on bioluminescence resonance energy transfer from firefly luciferase." J. Biosci. Bioeng. 93(6), 537-542 (2002) [Abstract] [PDF]

40. Yokozeki, T., H. Ueda*, R. Arai, W. Mahoney and T. Nagamune. "A homogeneous noncompetitive immunoassay for the detection of small haptens." Anal. Chem. 74(11), 2500-2504 (2002) [Abstract] [PDF]

39. Arai, R., H. Nakagawa, K. Tsumoto, W. Mahoney, I. Kumagai, H. Ueda* and T. Nagamune*. "Demonstration of a homogeneous noncompetitive immunoassay based on bioluminescence resonance energy transfer." Anal. Biochem. 289(1), 77-81 (2001) [Abstract]

38. Arai, R., H. Ueda, A. Kitayama, N. Kamiya and T. Nagamune. "Design of the linkers which effectively separate domains of a bifunctional fusion protein."
Protein Eng. 14(8), 529-532 (2001) [Abstract]

37. Kawahara, M., A. Natsume, S. Terada, K. Kato, K. Tsumoto, I. Kumagai, M. Miki, W. Mahoney, H. Ueda* and T. Nagamune. "Replacing factor dependency with that for lysozyme: affordable culture of IL-6 dependent hybridoma by transfecting artificial cell surface receptor." Biotechnol. Bioeng. 74(5), 416-423 (2001) [Abstract]

36. Kawahara, M., H. Ueda*, K. Tsumoto, I. Kumagai, W. Mahoney and T. Nagamune. "A growth signal with an artificially induced erythropoietin receptor-gp130 cytoplasmic domain heterodimer." J. Biochem. 130(2), 305-312 (2001) [Abstract]

35. Ura, S., H. Ueda, J. Kazami, G. Kawano and T. Nagamune. "Single cell reporter assay using cell surface displayed Vargula luciferase." J. Biosci. Bioeng. 92(6), 575-579 (2001) [Abstract] [PDF]

34. Zako, T., K. Harada, T. Mannen, S. Yamaguchi, A. Kitayama, H. Ueda and T. Nagamune. "Monitoring the refolding process of immobilized firefly luciferase using a biosensor based on surface plasmon resonance."
J. Biochem. 129(1), 1-4 (2001) [Abstract]

Reviews & Book chapters

Ueda, H. "Open sandwich immunoassay: a novel immunoassay approach based on the interchain interaction of an antibody variable region." J. Biosci. Bioeng. 94(6), 614-619 (2002) [Abstract] [PDF]