論文・著書:2001-3

51. Aburatani, T., K. Sakamoto, K. Masuda, K. Nishi, H. Ohkawa, T. Nagamune and H. Ueda. "A general method to select antibody fragments suitable for noncompetitive detection of monovalent antigens." Anal. Chem. 75(16), 4057-4064 (2003) [Abstract][PDF]

50. Kamiya, N., T. Takazawa, T. Tanaka, H. Ueda and T. Nagamune. "Site-specific crosslinking of functional proteins by transglutamination." Enzyme Microb. Tech. 33, 492-496 (2003)

49. Kawahara, M., H. Ueda, S. Morita, K. Tsumoto, I. Kumagai and T. Nagamune. "Bypassing antibiotic selection: positive screening of genetically modified cells with an antigen-dependent proliferation switch." Nucleic Acids Res. 31(7), e32 (2003) [Abstract][PDF]

48. Kitayama, A., H. Yoshizaki, Y. Ohmiya, H. Ueda* and T. Nagamune. "Creation of a thermostable firefly luciferase with pH-insensitive luminescent color." Photochem. Photobiol. 77(3), 333-338 (2003) [Abstract]

47. Ueda, H., T. Yokozeki, R. Arai, K. Tsumoto, I. Kumagai and T. Nagamune. "An optimized homogeneous noncompetitive immunoassay based on the antigen-driven enzymatic complementation." J. Immunol. Methods 279, 209-218 (2003) [Abstract]

46. Zako, T., K. Ayabe, T. Aburatani, N. Kamiya, A. Kitayama, H. Ueda* and T. Nagamune. "Luminescent and substrate binding activities of firefly luciferase N-terminal domain." Biochim. Biophys. Acta 1649, 183-189 (2003) [Abstract]

45. Aburatani, T., H. Ueda* and T. Nagamune. "Importance of a CDR H3 basal residue in VH/VL interaction of human antibodies." J. Biochem. 132(5), 775-782 (2002) [Abstract]

44. Arai, R., H. Nakagawa, A. Kitayama, H. Ueda and T. Nagamune. "Detection of protein-protein interaction by bioluminescence resonance energy transfer from firefly luciferase to red fluorescent protein." J. Biosci. Bioeng. 94(4), 362-364 (2002) [Abstract] [PDF]

43. Kawahara, M., H. Ueda, K. Tsumoto, I. Kumagai, W. Mahoney and T. Nagamune. "Selection of highly productive mammalian cells based on an inducible growth advantage using an antibody/receptor chimera." J. Biosci. Bioeng. 93(4), 399-404 (2002) [Abstract] [PDF]

42. Ohiro, Y., R. Arai, H. Ueda and T. Nagamune. "A homogeneous and noncompetitive immunoassay based on the enhanced fluorescence resonance energy transfer by leucine zipper interaction." Anal. Chem. 74(22), 5786-5792 (2002) [Abstract]

41. Yamakawa, Y., H. Ueda*, A. Kitayama and T. Nagamune. "Rapid homogeneous immunoassay of peptides based on bioluminescence resonance energy transfer from firefly luciferase." J. Biosci. Bioeng. 93(6), 537-542 (2002) [Abstract] [PDF]

40. Yokozeki, T., H. Ueda*, R. Arai, W. Mahoney and T. Nagamune. "A homogeneous noncompetitive immunoassay for the detection of small haptens." Anal. Chem. 74(11), 2500-2504 (2002) [Abstract] [PDF]

39. Arai, R., H. Nakagawa, K. Tsumoto, W. Mahoney, I. Kumagai, H. Ueda* and T. Nagamune*. "Demonstration of a homogeneous noncompetitive immunoassay based on bioluminescence resonance energy transfer." Anal. Biochem. 289(1), 77-81 (2001) [Abstract]

38. Arai, R., H. Ueda, A. Kitayama, N. Kamiya and T. Nagamune. "Design of the linkers which effectively separate domains of a bifunctional fusion protein." Protein Eng. 14(8), 529-532 (2001) [Abstract]

37. Kawahara, M., A. Natsume, S. Terada, K. Kato, K. Tsumoto, I. Kumagai, M. Miki, W. Mahoney, H. Ueda* and T. Nagamune. "Replacing factor dependency with that for lysozyme: affordable culture of IL-6 dependent hybridoma by transfecting artificial cell surface receptor." Biotechnol. Bioeng. 74(5), 416-423 (2001) [Abstract]

36. Kawahara, M., H. Ueda*, K. Tsumoto, I. Kumagai, W. Mahoney and T. Nagamune. "A growth signal with an artificially induced erythropoietin receptor-gp130 cytoplasmic domain heterodimer." J. Biochem. 130(2), 305-312 (2001) [Abstract]

35. Ura, S., H. Ueda, J. Kazami, G. Kawano and T. Nagamune. "Single cell reporter assay using cell surface displayed Vargula luciferase." J. Biosci. Bioeng. 92(6), 575-579 (2001) [Abstract] [PDF]

34. Zako, T., K. Harada, T. Mannen, S. Yamaguchi, A. Kitayama, H. Ueda and T. Nagamune. "Monitoring the refolding process of immobilized firefly luciferase using a biosensor based on surface plasmon resonance." J. Biochem. 129(1), 1-4 (2001) [Abstract]



総説

上田宏 (2003). 新規免役測定法に適した抗体断片の取得. コンビナトリアルバイオエンジニアリング. 植田充美・近藤昭彦編., 化学同人. 9: 105-110.

上田宏. "簡便迅速な免役測定法の開発." テクノニュース千葉 29(1), 8-9 (2003)

河原正浩, 上田宏, 長棟輝行. "抗体を用いた受容体のエンジニアリングー効果的な細胞医療を目指してー" バイオインダストリー 20(7), 23-33 (2003)

上田宏, 大川秀郎, 長棟輝行. "競争のいらない小分子の高感度免役測定法の開発." バイオインダストリー 20(7), 52-59 (2003)

Ueda, H. "Open sandwich immunoassay: a novel immunoassay approach based on the interchain interaction of an antibody variable region." J. Biosci. Bioeng. 94(6), 614-619 (2002) [Abstract] [PDF]

上田宏. "ファージディスプレイ技術による機能性分子の選択と創製." 生物工学会誌 79(9), 354-357 (2001) [PDF]

吉村徹, 片倉啓雄, 上田宏. "タンパク質のファージディスプレイ法." 現代化学(367), 55-62 (2001)

河原正浩, 上田宏, 長棟輝行. "無血清・低血清培地で増殖する動物細胞の創出." バイオサイエンスとインダストリー 59(9), 35-36 (2001)