157. J. Su, T. Kitaguchi, Y. Ohmuro-Matsuyama, T. Seah, F.J. Ghadessy, S. Hoon and H. Ueda "Transmembrane signaling on a protocell: Creation of receptor-enzyme chimeras for immunodetection of specific antibodies and antigens" Sci. Rep. 9, 18189 (2019).[DOI] Open Access
156. Y. Cong, H. Dong, X. Wei, L. Zhang, J. Bai, J. Wu, J.X. Huang, Z. Gao, H. Ueda and J. Dong "A novel murine antibody and an open sandwich immunoassay for the detection of clenbuterol" Ecotoxicol. Environ. Saf. 182, 109473 (2019).[DOI]
155. C.-I. Chung, Y. Sato, Y. Ohmuro-Matsuyama, S. Machida, H. Kurumizaka, H. Kimura and H. Ueda "Intrabody-based FRET probe to visualize endogenous histone acetylation" Sci. Rep. 9, 10188 (2019). [DOI] Open Access
154. J. Su, C. Beh, Y. Ohmuro-Matsuyama, T. Kitaguchi, S. Hoon and H. Ueda "Creation of stable and strictly regulated enzyme switch for signal-on immunodetection of various small antigens" J. Biosci. Bioeng. 128, 677-682 (2019).[DOI]
153. R. Dale, Y. Ohmuro-Matsuyama, H. Ueda and N. Kato "Non-steady state analysis of enzyme kinetics in real time elucidates substrate association and dissociation rates: demonstration with analysis of firefly luciferase mutants" Biochemistry 58, 2695-2702 (2019). [DOI]
152. M. Mita, M. Ito, K. Harada, I. Sugawara, H. Ueda, T. Tsuboi and T. Kitaguchi "Green fluorescent protein-based glucose indicators report glucose dynamics in living cells" Anal. Chem. 91, 4821-4830 (2019). [DOI]
151. H.J. Jeong, J. Dong and H. Ueda "Single-step detection of influenza virus hemagglutinin using bacterially produced Quenchbodies" Sensors 19, 52 (2019). [DOI] Open Access
150. A. Sasao, M. Takaki, H.-J. Jeong, K. Yonemitsu, Y. Ohtsu, H. Tsutsumi, S. Furukawa, H. Morioka, H. Ueda and Y. Nishitani "Development of a fluvoxamine detection system using a Quenchbody, a novel fluorescent biosensor" Drug Test. Anal. 11, 601-609 (2019). [DOI]
149. Y. Ohmuro-Matsuyama, T. Yamashita, K. Gomi, H. Yamaji and H. Ueda "Evaluation of protein-ligand interactions using the luminescent interaction assay FlimPIA with streptavidin-biotin linkage" Anal. Biochem. 563, 61-66 (2018). [DOI]
148. J. Dong, R. Fujita, T. Zako and H. Ueda "Construction of Quenchbodies to detect and image amyloid β oligomers" Anal. Biochem. 550, 61-67 (2018). [DOI]
147. S. Zhao, J. Dong, H.-J. Jeong, K. Okumura and H. Ueda "Rapid detection of the neonicotinoid insecticide imidacloprid using a quenchbody assay" Anal. Bioanal. Chem. 410, 4219-4226 (2018). [DOI]
146. J. Su, D. Jinhua, T. Kitaguchi, Y. Ohmuro-Matsuyama and H. Ueda "Noncompetitive homogeneous immunodetection of small molecules based on beta-glucuronidase complementation" Analyst 143, 2096-2101 (2018).[DOI]
145. Y. Ohmuro-Matsuyama and H. Ueda "Homogeneous noncompetitive luminescent immunodetection of small molecules by ternary protein fragment complementation" Anal. Chem. 90, 3001-3004 (2018).[DOI] [PDF–ACS Articles on Request]
144. H.-J. Jeong$, T. Kawamura$, M. Iida, Y. Kawahigashi, M. Takigawa, Y. Ohmuro-Matsuyama, C.-I. Chung, J. Dong, M. Kondoh and H. Ueda "Development of a Quenchbody for the detection and imaging of the cancer-related tight-junction-associated membrane protein claudin" Anal. Chem. 89, 10783-10789 (2017). [DOI] $Equal contributions
143. M. Okochi, M. Muto, K. Yanai, M. Tanaka, T. Onodera, J. Wang, H. Ueda and K. Toko "Array-based rational design of short peptide probe derived from anti-TNT monoclonal antibody" ACS Comb. Sci. 19, 625-632 (2017). [DOI]
142. H.-J. Jeong, K. Matsumoto, S. Itayama, K. Kodama, R. Abe, J. Dong, M. Shindo and H. Ueda Construction of dye-stapled Quenchbody by photochemical crosslinking to antibody nucleotide-binding site" Chem. Commun. 53, 10200-10203 (2017). [DOI]
141. Y. Ohmuro-Matsuyama, T. Yamashita, H. Lin, H. Yamaji and H. Ueda "Improved sensitivity of firefly luminescent intermediate-based protein interaction assay using Ser 440 mutant with lower adenylation activity" Luminescence 33, 125-130 (2018).[DOI]
140. D. Wongso, J. Dong, H. Ueda and T. Kitaguchi "Flashbody: a next generation Fluobody with fluorescence intensity enhanced by antigen binding" Anal. Chem. 89, 6719-6725 (2017).[DOI]
139. J. Dong, M. Shichiri, C.-I. Chung, T. Shibata, K. Uchida, Y. Hagihara, Y. Yoshida and H. Ueda "An Open Sandwich immunoassay for detection of 13(R,S)-Hydroxy-9(E),11(E)-octadecadienoic acid" Analyst 142, 787-793 (2017).[DOI]
138. H.-J. Jeong, T. Kojima, J. Dong, H. Ohashi and H. Ueda "One-pot construction of Quenchbodies using antibody-binding proteins" Anal. Methods 8, 7774-7779 (2016).[DOI]
137. C.-I. Chung, R. Makino, Y. Ohmuro-Matsuyama and H. Ueda "Development of a fluorescent protein-antibody FRET probe for the detection and imaging of osteocalcin" J. Biosci. Bioeng. 123, 272-276 (2017). [DOI]
136. H. Ohashi, T. Matsumoto, H.-J. Jeong, J. Dong, R. Abe and H. Ueda "Insight into the working mechanism of Quenchbody: Transition of the dye around antibody variable region that fluoresces upon antigen binding" Bioconj. Chem. 27, 2248-2253 (2016). [DOI] [PDF–ACS Articles on Request
135. H. Iwai, M. Kojima-Misaizu, J. Dong, and H. Ueda "Creation of a ligand-dependent enzyme by fusing circularly permuted antibody variable region domains" Bioconj. Chem. 27, 868-873 (2016). [ DOI] [Abstract] [PDF–ACS Articles on Request]
134. Y. Ohmuro-Matsuyama, K. Mori, H. Hamada, H. Ueda and H. Yamaji "Electrostatic engineering of the interface between heavy and light chains promotes antibody Fab fragment production" Cytotechnol. 69 (3), 469-475 (2017).[DOI]
133. R. Dale, Y. Ohmuro-Matsuyama, H. Ueda and N. Kato "Mathematical model of the firefly luciferase complementation assay reveals a non-linear relationship between the detected luminescence and the affinity of the protein pair being analyzed" PLoS ONE 11, e0148256 (2016). [DOI][Abstract] Open Access
132. J. Dong, H.J. Jeong and H. Ueda "Preparation of Quenchbodies by protein transamination reaction" J. Biosci. Bioeng. 122, 125-130 (2016). [DOI][Abstract]
131. H.J. Jeong, T. Kawamura, J. Dong and H. Ueda "Q-bodies from recombinant single-chain Fv fragment with better yield and expanded palette of fluorophores" ACS Sens. 1, 91-99 (2016).[DOI]
130. M. Kurihara$, Y. Ohmuro-Matsuyama$, K. Ayabe, T. Yamashita, H. Yamaji and H. Ueda "Ultra sensitive firefly luciferase-based protein-protein interaction assay (FlimPIA) attained by hinge region engineering and optimized reaction conditions" Biotechnol. J. 11, 91-99 (2016). [Abstract][DOI] $Equal contributions
総説・著書
董 金華，上田 宏， "がん以外には結合させない治療用抗体の改造技術" 実験医学 37(11), 3100-1 (2019).[Link]
大室有紀，上田 宏，"分割NanoLucを用いた相互作用検出系による低分子の免疫測定" 実験医学別冊 発光イメージング実験ガイド (Eds. 永井健治・小澤岳昌）, 194-197 (2019).[Link]
Yuki Ohmuro-Matsuyama and Hiroshi Ueda "Protein-Protein Interaction Assays Using Split-NanoLuc" in Bioluminescence (ed. H. Suzuki) (IntechOpen, London; 2019). [Link] Open Access
上田 宏，"オープンサンドイッチ原理に基づく免疫センサープローブの構築" 生物化学的測定研究会年報 23, (1) 187-193 (2019).[Link]
上田 宏，"タンパク工学とケミカルバイオロジーによる免疫センサープローブの構築" 日本化学会バイオテクノロジー部会ニュースレター 22, (2) 3-11 (2019).[Link]
上田 宏， "2018年ノーベル化学賞を受賞したGregory Winter博士" 現代化学 573, 26-27 (2018).[Link]
Yuki Ohmuro-Matsuyama and Hiroshi Ueda "A Novel Protein-Protein Interaction Assay Based on the Functional Complementation of Mutant Firefly Luciferases: Split Structure Versus Divided Reaction" in Protein-Protein Interaction Assays (ed. Mahmood-Ur-Rahman Ansari) pp. 11-27 (IntechOpen, London; 2018). [Link] Open Access
董 金華，上田 宏， "転写因子IRF4の欠失によるCD4+ T細胞の機能不全が移植受容を促進する" 実験医学 36(4), 546-7 (2018).[Link]
Jinhua Dong and Hiroshi Ueda "Open sandwich immunoassay and its applications" in Advances in Medicine and Biology, 125. (ed. L.V. Berhardt) pp. 123-159 (Nova Science Publishers Inc., New York; 2017). [Link][PDF (PW:392062)]
董 金華，上田 宏， "FRETセンサーを用いた生細胞膜電位の定量的測定" 実験医学 35(9), 1464-5 (2017).[Link]
H. Iwai, M. Kojima-Misaizu, J. Dong and H. Ueda "Creation of antigen-dependent β-lactamase fusion protein tethered by circularly permuted antibody variable domains" Methods Mol. Biol. 1596, 149-165 (2017). [DOI]
Y. Ohmuro-Matsuyama and H. Ueda "Ultrasensitive Firefly luminescent intermediate-based Protein-protein Interaction Assay (FlimPIA) based on the functional complementation of mutant firefly luciferases" Methods Mol. Biol. 1596, 119-130 (2017). [DOI]
Jinhua Dong and Hiroshi Ueda "ELISA-type assays of trace biomarkers using microfluidic methods" WIREs Nanomed. Nanobiotechnol. e1457 (2017). [Abstract]
Y. Ohmuro-Matsuyama and H. Ueda "A protein-protein interaction assay FlimPIA based on the functional complementation of mutant firefly luciferases" " Methods Mol. Biol. 1461, 131-142 (2016). [DOI]
上田 宏, 董 金華, 鄭 熙陳, 阿部 亮二 "ネオバイオ分子としてのQ-body" 生物工学 94(8), 489-91 (2016). [Link]
董 金華，上田 宏， "CRISPR/Cas9システムによるマウス・ヒト抗体遺伝子座の編集" 実験医学 34(9), 1422-3 (2016).[Link]
上田 宏 "抗原結合により光る抗体Q-bodyの創出" 細胞 48, 23-27 (2016). [Link]
上田 宏, 阿部 亮二 "「その場」検出を可能とする光る抗体Q-body" 検査技術 21, 9-15 (2016). [Link]
上田 宏，抗体のセンサー化で生命現象を探る" 日経バイオテク12月21日号「バイオイメージング最前線」（第8回）(2015). [PDF]
董 金華，上田 宏， "生物発光タンパク質センサーを用いた薬剤のモニタリング" 実験医学 33(4), 583-584 (2015).
阿部 亮二, 上田 宏 "抗体断片の蛍光技術を用いた迅速・簡便な検査キットの開発" 「最先端バイオマーカーを用いた診断薬／診断装置開発と薬事対応」 p. 335-338（株）技術情報協会, 東京，2015. [Link]
生物化学的測定研究会，小林典裕，上田 宏，三宅司郎，荒川秀俊編「免疫測定法〜基礎から先端まで」2.3、4.3、6.4、6.5、7.5章 講談社サイエンティフィック[Link]
H. Ueda and J. Dong "From Fluorescence Polarization to Quenchbody : Recent Progress in Fluorescent Reagentless Biosensors Based on Antibody and Other Binding Proteins" Biochim. Biophys. Acta - Proteins and Proteomics 1844, 1951-1959 (2014).[Abstract][DOI]
上田 宏 “免疫反応を測れる 光る抗体” 現代化学５月号 p. 32-36 (2014)
大室有紀，上田 宏 ”ホタル発光酵素の反応機構を利用したタンパク質間相互作用検出系の開発” バイオサイエンスとインダストリー 72(2), 113-116 (2014).
招待講演
上田 宏, 安田 貴信, 上野 慶行, 井上 暁人,髙橋 昌樹, 大室 有紀, 北口 哲也, 董 金華 "Beyond Q-body: 色素修飾が不要な新規免疫測定素子の構築",
第71回日本生物工学会大会シンポジウム「産業化を目指した生体分子工学の新たな潮流」, 岡山大学津島キャンパス，岡山 (9/16, 2019)
上田 宏 "理想の免疫測定を目指した 抗体タンパク質のセンサー化戦略",
日本分析化学会第68年会シンポジウム「タンパク質を素材とする分析ツールの進化デザイン」, 千葉大学西千葉キャンパス，千葉 (9/13, 2019)
上田 宏 "蛋白質工学の基礎技術：蛋白質機能の設計と分子進化",
第19回日本蛋白質科学会年会 第71回日本細胞生物学会大会 合同年次大会, 神戸国際会議場，神戸 (6/25, 2019)
上田 宏 "ノーベル化学賞を受賞したイノベーター： Gregory Winter博士",
2019年度 日本生化学会関東支部例会, 東工大すずかけ台キャンパス，横浜 (6/22, 2019)
上田 宏 "均相系測定を可能にする抗原応答性標識抗体の創製",
日本分析化学会第67年会, 東北大学川内キャンパス，仙台 (9/12, 2018)
Yuki Ohmuro, Hiroshi Ueda " Novel Protein-Protein Interaction Assay Based on the Functional Complementation of Mutant Firefly Luciferases: Split Structure Versus Divided Reaction",
第18回日本蛋白質科学会年会, 朱鷺メッセ，新潟 (6/26, 2018)
上田 宏 "新規免疫測定法開発におけるBLIの活用例",
日本ポール-ForteBio User Meeting 2018, ANAインターコンチネンタルホテル東京 (5/8, 2018)
上田 宏 "抗体結合タンパク質の最適な多価化による活性向上と、蛍光免疫センサー構築への応用",
埼玉大学先端産業国際ラボラトリー メディカルイノベーション研究ユニット(MiU)第 4 回ワークショップ
"多価化が拓くイノベーション", 埼玉大学, さいたま (11/2, 2017)
上田 宏 “蛍光消光現象と発光酵素を利用した新原理免疫測定法の開発", 第112回岡山県医用工学研究会
平成29年度第2回セミナー・交流会 "抗体応用への生物工学的アプローチ” 岡山大学鹿田キャンパス (10/27, 2017)
H. Ueda “Development of antibody-based imaging probes utilizing fluorescence quenching phenomena",
International Conference on Translational Medicine and Imaging (ICTMI) 2017,
Vellore Institute of Technology, Vellore, India (8/30, 2017)
H. Ueda “How can we transform monofunctional proteins to sensors?”,
Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences,
Nanyang Technological University, Singapore (3/14, 2017)
H. Ueda “How can we convert antibody molecule to a biosensor?”,
5th Bioscience and Biotechnology International Symposium,
Tokyo Tech Yokohama (1/11, 2017)
上田 宏 「抗体・酵素のエンジニアリングによる新規検出法の創成」, 東京工業大学生命理工学院第一回学院セミナー, 東工大すずかけキャンパス (7/20, 2016)
Hiroshi Ueda, Yuya Oka, Chan-I Chung, Hee-Jin Jeong, Jinhua Dong “Antibody-based Fluorescent Probes: How Can We Convert Antigen Binding Signal to Fluorescence Signal?”, Third FB3 (Fluorescent Biomolecules and their Building Blocks) Conference, Tianjin University, Tianjin, People's Republic of China (7/8, 2016)
上田 宏. 「組み換え抗体を用いた酸化脂質検出系の構築」，第11回レドックス・ライフイノベーションシンポジウム（お茶の水女子大学）(3/17, 2016)
Hiroshi Ueda “Development of biosensor proteins utilizing fluorescence quenching and bioluminescent reactions”, 6th International Conference on Bimolecular Engineering, Society for Biological Engineering, AIChE, Grand Hyatt Singapore, Singapore (1/5, 2016)
